Handling Guides

Application Note – Spiral Sorter Chip Fluidic 382

In this application note, we introduced a commercially-available microfluidic system to perform passive size separation of a microparticle mixture. The system includes a spiral-shaped microfluidic device from microfluidic ChipShop and pressure-based flow controllers from Fluigent. Tuning Dean and lift forces induced by spiral microchannels allow to obtain distinct particle streams and subsequently sort particles according to their sizes.

Fluidic 382 – Spiral Sorter

Pump system from Fluigent used for the spiral sorter experiment

Find the Application Note here:

Application Note for Fluidic 382

(PDF document, 1905 KB)

Application Note – Herringbone Mixer Fluidic 1460

We have designed the Herringbone Mixer chip Fluidic 1460 with two different channel geometries. Due to the uniformly arranged mixing structures, the synthesis of lipid nanoparticles can be tailored to a defined size. In this application note, the synthesis of lipid nanoparticles is explained and described in a protocol.

Herringbone Mixer Fluidic 1460

Herringbone Mixer Fl. 1460 mixes two liquids

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Application Note for Fluidic 1460

Handling Instructions – 3D Serpentine Mixer Fluidic 1079

Many microfluidic systems require rapid mixing of different fluids within microchannels. While diffusion can be an effective mode of mixing in microchannels with predominantly laminar flows, it often requires prolonged mixing times and/or ideally increase of interfacial area.
Introducing chaotic (but still laminar) flow patterns can aid mixing efficiency in microchannels. This strategy becomes even more important when mixing solutions with particularly low diffusion coefficients. The 3D serpentine mixer has been developed especially to address this challenge in microfluidics. It is the perfect tool to investigate fast and efficient passive micromixing of up to three liquids.

Fluidic 1079 – 3D Serpentine Mixer

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Handling Instructions for Fluidic 1079

(PDF document, 1074 KB)

Handling Instructions – Diffusion Mixer Fluidic 186

As flows in microchannels are usually laminar, mixing of liquids at microscale is a challenge but can be tackled using various approaches. One of the simplest methods for effective micromixing is to increase the interfacial areas between the fluids. Diffusion will cause the transfer of molecules through the interfacial area.
microfluidic ChipShop‘s diffusion mixer with its 217 mm long channel that enables a prolonged co-flow of fluids, makes use exactly of this principle.

Fluidic 186 – Diffusion mixer

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Handling Instructions for Fluidic 186

(PDF document, 1033 KB)

Handling Instructions – 2D Gradient Generator

In life science, tight control of the chemical environment is the basis for many experiments. A high spatial and timed resolution is key to successful experiments relying on gradients.
For this reason, we introduce the 2D Gradient Generator Fluidic 1166. It enables you to create gradients with up to six different fluids while at the same time allowing high control of flow and diffusion rates.

 Fluidic 1166 – 2D Gradient Generator

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Handling Instructions for Fluidic 1166

(PDF document, 754 KB)

Handling Instructions – Channel Interaction Chip 983

With the interaction chip family cell-cell interaction, of cells cultured in separate culture compartments, can be monitored. The channel interaction chip Fluidic 983, has been developed to study cells co-cultured in three adjacent channels, to each of which a microfluidic flow can be applied.

Fluidic 983 – Channel Interaction Chip

Find the Handling Instruction here:

Handling Instructions for Fluidic 983

(PDF document, 1320 KB)

Handling Instructions – Cell Trap Chip 913

The isolation of individual cells can be particularly helpful in the analysis of cells and their communication with each other. For this reason, the cell trap chip Fl. 913 was developed. It enables you to collect individual cells and particles in wells where they can be monitored directly.

Fluidic 913 – Cell Trap Chip

Find the Handling Instruction here:

Handling Instructions for Fluidic 913

(PDF document, 2600 KB)

General Handling Guide – Open Chip Platforms for Self-Assembly

The straight channel chip with double sided adhesive tape represents a microfluidic chip system for self-assembly that enables customized reagent and assay integration for various kinds of applications. For these purposes several chip types are at hand, having one or more integrated fluidic channels that remain open for manipulation at customer’s side.

Straight channel chip Fluidic 268 – how to remove the protective film

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General Handling Guide – Open Chip Platforms for Self-Assembly

(PDF document, 588 KB)

Droplet generation

Droplet generation using microfluidics has attracted a lot of interest due to the ability to produce highly monodispersed droplets with high frequency. The generation of droplets is based on the use of two immiscible phases: continuous phase (medium in which droplets flow) and dispersed phase (the droplet).

Overview on droplet generator chips, accessories, pump setups, kits and droplet oils

Droplet generator chip Fluidic 1032 – Multi channel design with double-cross geometry

Find out what microfluidic ChipShop has to offer to help you in successfully setting up your droplet generation experiment:

Overview on droplet generation

(PDF document, 3220 KB)

Application Note for droplet generation using Fluidic 440 and a microfluidic system from Fluigent

Droplet generator chip Fluidic 440 – used for the Application note from Fluigents microfluidic system.

Our droplet generator Fluidic 440 shows size variation of one main structure for the evaluation of generated droplet size under the desired conditions. Fluidic 440 is designed to generate droplets with channel dimensions at the droplet formation region of 80 μm, 70 μm, 60 μm and 50 μm channel width and height.

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Production of water-in-oil emulsions using a droplet generator chip

(PDF document, 680 KB)

User Manual for ChipGenie® edition TSO

The ChipGenie® edition TSO allows for the temperature control of microfluidic chips in microscope slide format. A fixed temperature as well temperature cycling can be done to enable e.g. a fast PCR on chip. The ChipGenie® edition TSO is equipped with a fluorescence-based read-out unit to carry out the optical detection on microfluidic ChipShop’s chamber chips following the spacing of a 384 well plate of 4.5 mm with fixed read-out positions.

Reaction chamber chip Fluidic 584 – suited to be used for heatcycle applications, such as PCR experiments using the ChipGenie® edition T.

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ChipGenie® edition TSO

(PDF document, 1.80 MB)

Flyer for ChipGenie® edition Dx

The ChipGenie® edition Dx is a customized diagnostic platform and its respective cartridge. The user chooses the sample and detection method. The ChipGenie® edition Dx is a fully automized point-of-care device working in a bleed-to-read fashion.

Cartridge for the ChipGenie® edition Dx platform.

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ChipGenie® edition Dx

(PDF document, 983 KB)