The double emuslion droplet chip Fluidic 1480 provides two droplet generator units with double-cross geometry, that have varying channel/nozzle sizes at the second cross. It comes with a specific surface coating that allows for the generation of double emulsions, such as the inclusion of droplets or cells deriving from the first channel intersection in a further droplet shell at the second channel intersection. Each unit consists of a two crossings, each with a flow-focusing geometry, that are connected by a meander channel to generate W/O/W emulsions in a single step. To generate water-in-oil droplets in an aqueous continuous liquid, the chips are provided with a partially hydrophilic coating. However, this chip can also be used without pretreatment to mix two aqueous phases through the meander structures and to generate W/W/O droplets. Fluidic 1480 features standard Mini Luer interfaces to seamlesly connect to a pump system of choice. For double emulsion experiments, at least three individual pump channels are required.

Application Note Fluidic 1480