This integrated chip allows for the development of biological assays on chip. The chip enables for on-chip metering, mixing, and the detection of the reaction in a separate chamber.
Assay Chip 1 – On Board Metering, Mixing, and Reaction
Assay Chip 2 – Turning Valve Assisted Fluid Control with Separate Assay and Reference Cavities
This integrated chip allows for the development of hybridization assays on chip. Six 12 μl cavities with 200 μm depth can be used for spotting different kinds of arrays. Four of these chambers are operated in row, two further separately. The separate chambers allow e.g. for control or quantification reactions and have pre-cavities to store dry reagents. Sample injection is foreseen through a female Luer interface, reagent supply or air pressure through the female Mini Luer interfaces.
Assay Chip 3 – Assay Development Chip for Magnetic Bead Based or Hybridization Assays
A 300 μm deep central chamber with 30 μl inner volume is the reaction cavity of this chip. To keep the components in the chamber either magnetic beads can be used which need to be operated by an external magnet or catcher molecules have to be immobilized on the surface of the cavity. Controlled by two turning valves liquids can be supplied and removed, air pressure can be applied and venting can be ensured. Liquid supply and air pressure are foreseen through the female Mini Luer interfaces on chip.
Assay Chip 4
This chip is an assay chip e.g. for cell-based assays that can be fluidically operated using hydrostatic pressure generated by attached liquid filled tanks.