With this system, which comprises an 8-channel high frequency signal generator (DEP1) and a microfluidic chip (DFC1) with integrated electrodes, single suspension cells can be trapped (up to two at a time) in a laminar flow of a given aqueous solution without any physical contacts to solid objects. An ensemble of eight microelectrodes (an electric field cage) produces a high-frequency electromagnetic field that acts on the cells and forces them with micrometer precision to a defined position in the micofluidic channel of the chip. The forces acting on the cells are sufficiently strong to maintain the position of the cell against the flow of the solution in the channel. By adding a reagent of interest (ligands, antibodies, signal molecules etc.) to the solution, the cell can be exposed to the reagent with high temporal resolution while the cellular response to it can be monitored by optical microscopy. As tested under various experimental conditions, cell viability is maintained for hours, under optimal conditions even for up to days.
DropBot by Sci-Bots is a portable, general-purpose Digital Microfluidic control system that can be used to manipulate discrete droplets using electrostatic forces on an insulated array of electrodes; a format also commonly referred to as Electrowetting on a Dielectric (EWOD). This small and rugged instrument can be controlled via USB with easy-to-use software that supports graphical programming (i.e., users can simply click and drag drops using a real-time video overlay). Sequences of steps can be pre-programmed and run automatically, enabling fully automated operation
- Integrated high-voltage source (up to 140 VRMS bipolar square waves at frequencies between 100 Hz–10 kHz)
- 120 independent channels connected to the chip via spring loaded pogo-pins
- Dynamic impedance sensing providing real-time measurement of drop position/velocity
- Extensible software supports easy integration/control of new sensors and actuators via plugins
- Sample preparation
- Chemical synthesis
- Cell-based assays
- Adherent 3D cell culture
Active Microfluidic Mixing with MXR from Redbud Labs
MXR by Redbud Labs – Make any chamber a mixing chamber: MXR adds active mixing to microfluidic systems. Using a combination of pumping and chaotic advection, MXR gently agitates volumes from 1–100 μL. MXR is ideal for enhancing assays that are limited by mass transport, including:
- Hybridization and microarray incubation
- Mixing of liquids and resuspension of dried reagents
- Fluid exchange
MXR can be ordered pre-installed, or as a pop-in module. A simple peel-and-stick operation adds MXR to your chip.
MXR is powered by Redbud Posts – millions of flexible, magnetic micro-rods that act as tiny stirbars. To learn more about MXR and Redbud Post technology, visit redbudlabs.com.
MXR Drivers – The Redbud STAGE
For temperature controlled assays, Redbud Stage enables simultaneous mixing and incubation of up
to two MXR-enabled chips, with additional room to pre-heat reagents and set aside non-mixing control
chambers. As your assay develops, Redbud Labs can design customized inserts to fully integrate the
drive system with your instrumentation. A smaller MXR driver for applications where the heated stage is
not necessary is available. Kindly contact us for more information.
Customize any of microfluidic ChipShop’s chips with MXR
Please contact us to learn how MXR can be customized to fit your application. MXR can be integrated into any of microfluidic ChipShop’s wide variety of microfluidic solutions.