The field-flow fractionation chip Fluidic 120 features olive interfaces and field-flow fractionation structure. The chip was developed within the BMBF-Project “Free-Flow-Chip”, FKZ 01RI0643D, and can be used to establish a miniaturized free-flow electrophoresis (FFE).
In free-flow electrophoresis, charged analytes entering the separation bed are deflected to the side by the electric field applied at right angles to the direction of flow, causing anions to migrate to the anode (positive pole) and cations to the cathode (negative pole). The combination of laminar flow and electrophoretic migration at a 90° angle to it forms a deflection angle, which depends on the electrophoretic mobility of the analyte in question and the applied flow rates and field strengths.
Please note: This product only contains the plastic chip as platform to establish your experiment. It does not contain electrodes.
For more information on miniaturized free-flow electrophoresis using this chip, check out our publication: S. Köhler et al. RSC Adv., 2012,2, 520-525.