This reaction chamber chip with an arrangement of chambers having the same dimensions besides the cavity depth and therefore different volumes allow for the direct comparison of the chamber depth influence on the assay and the measurement. This chip assists in the choice of the right chamber dimensions in the design process of integrated microfluidic devices. Chamber volumes vary from 1.2 µl to 10.2 µl respectively 100 µm to 800 µm channel height. For a particularly convenient handling, this chip features pipetting holes